Flow-Dependent Regulation of Nitric Oxide Formation in the Isolated Canine Mesenteric Arterial Bed

The Japanese Journal of Pharmacology

Koichi Nakayama¹  Kunio Ishii¹  Yoshio Tanaka¹  Tsutomu Nakahara¹ 

[1] Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka

关键词: Canine mesenteric artery;    Flow;    Nitric oxide (NO);    NG-nitro-L-arginine;    Perfusion pressure;   

DOI  :  10.1254/jjp.74.275

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(24)Cited-By(2)Effects of flow rate changes on nitric oxide (NO)formation in vascular endothelial cells were investigated in isolated canine mesenteric arterial bed preparations. Stepwise increases in the flow rate from 8 ml/min to 40 ml/min significantly (P G-nitro-L-arginine (L-NNA, 100 μM), perfusion pressures were significantly (P<0.01)higher than those observed under control conditions at all flow rates examined. Sodium nitroprusside (SNP)(0.1-10 μM)counteracted the pressor effect of L-NNA in a concentration-dependent manner. Increases in the flow rate from 10 ml/min to 40 ml/min significantly (P < 0.05)augmented cyclic GMP production in the vascular bed preparation. The flow-induced cyclic GMP response was significantly (P<0.05)attenuated by L-NNA (100 μM). These results demonstrate that 1)the amount of NO released from endothelial cells toward vascular smooth muscle cells can be semi-quantified with SNP, and 2)an increase in the flow rate stimulates NO formation in endothelial cells of resistance arteries, which may play an important part in regulating systemic blood pressure.

【 授权许可】

Unknown