Antagonistic Effect of YM022, an Antiulcer Agent in Rats, on Human Cholecystokinin (CCK)B/Gastrin Receptor

The Japanese Journal of Pharmacology

Akito Nishida¹  Hidenori Yazawa¹  Kazuo Honda¹  Yuji Saita¹  Akira Miyake¹  Tomonobu Koizumi¹ 

[1] Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd.

关键词: YM022;    Cholecystokinin (CCK)-8;    CCKB/gastrin receptor;    Calcium mobilization;    Stable expression;   

DOI  :  10.1254/jjp.71.307

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(24)Cited-By(3)We recently isolated a cDNA clone for the human cholecystokinin (CCK)B/gastrin receptor and permanently expressed this receptor cDNA in NIH-3T3 cells. [125I]CCK-8 specifically bound to the membrane of the transfectant, and this binding was displaced by unlabeled CCK-8 with an IC50 of 0.32 nM. Treatment of these cells with CCK-8 increased the intracellular Ca2+ concentration with an EC50 of 0.30 nM. Using these cells expressing functional human CCKB/gastrin receptors, we investigated the pharmacological properties of (R)-1-[2, 3-dihydro-l-(2''-methylphenacyl)-2-oxo-5-phenyl-1H-1, 4-benzodiazepin-3-yl]-3-(3-methylphenyl) urea (YM022), a potent and selective CCKB/gastrin receptor antagonist in rats. YM022 potently inhibited [125I] CCK-8 binding to the membrane with an IC50 of 55 pM and CCK-8-induced Ca2+ mobilization with that of 7.4 nM. On the other hand, its racemate and enantiomer more weakly inhibited this binding (IC50 of 110 pM and 11 nM, respectively) and Ca2+ mobilization (IC50 of 18 nM and 94 nM, respectively). These results indicate that YM022 stereoselectively recognizes the human CCKB/gastrin receptor as a potent antagonist and that the established transfectant is useful for characterization of human CCKB/gastrin-receptor ligands.

【 授权许可】

Unknown