Effect of Saiboku-to, an Antiasthmatic Herbal Medicine, on Nitric Oxide Generation from Cultured Canine Airway Epithelial Cells

The Japanese Journal of Pharmacology

Kimio Konno¹  Jun Tamaoki¹  Atsushi Chiyotani¹  Hisashi Takemura¹  Mitsuko Kondo¹ 

[1] First Department of Medicine, Tokyo Women's Medical College

关键词: Chinese medicine;    Airway epithelium;    Nitric oxide;    Asthma;   

DOI  :  10.1254/jjp.69.29

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(40)Cited-By(4)The effect of Saiboku-to (TJ-96), an antiasthmatic Kampo medicine, on the generation of nitric oxide (NO) from cultured canine tracheal epithelium was investigated using a highly specific amperometric sensor for this molecule in vitro. Immersion of the NO-selective electrode in the medium containing tracheal epithelial cells detected the baseline current of 16.8 - 57.0 pA, which corresponded to an NO concentration ([NO]) of 39.7 ± 8.1 nM. Addition of TJ-96 increased [NO] in a concentration-dependent manner, the maximal increase from the baseline level and the concentration of TJ-96 required to produce a half-maximal effect (EC50) being 127.5 ± 20.1 nM (P < 0.001) and 86 ± 9 μg/ml, respectively. Pretreatment of cells with NG-nitro-L-arginine methylester (L-NAME) greatly inhibited the TJ-96-induced increase in [NO], whereas NG-nitro-D-arginine methylester (D-NAME) had no effect, and this inhibition was reversed by L-arginine but not by D-arginine. Cytochemical staining of the epithelial cells showed marked reactivity of NADPH diaphorase activity. These results suggest that NO is spontaneously released by the airway epithelium and that TJ-96 stimulates the epithelial NO generation.

【 授权许可】

Unknown