Potentiation of GABA-Induced Inhibition by 20-Hydroxyecdysone, a Neurosteroid, in Cultured Rat Cortical Neurons

The Japanese Journal of Pharmacology

Masashi Sasa¹  Hisamitsu Ujihara²  Kumatoshi Ishihara¹  Shuji Tsujiyama¹ 

[1]Department of Pharmacology, Hiroshima University School of Medicine

[2]Department of Pharmacology, School of Medicine, Yamaguchi University

关键词: GABA;    Neurosteroid;    Cultured cortical neuron;   

DOI  :  10.1254/jjp.68.133

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(10)Cited-By(9)Effects of 20-hydroxyecdysone (20-HE), a neurosteroid, on cultured rat cortical neurons were examined using the whole cell recording technique. Under the voltage and current clamp conditions, brief application (5 sec) of 20-HE alone did not produce current changes nor any changes in the membrane potential. However, the chemical dose-dependently potentiated the GABA-induced current and hyperpolarization, which were blocked by bicuculline. These results suggest that 20-HE acts on the modulatory site of the GABAA receptor and potentiates GABAergic inhibition in rat cortical neurons.

【 授权许可】

Unknown