Effects of Flavonoid Compounds on the Activity of NADPH Diaphorase Prepared from the Mouse Brain

The Japanese Journal of Pharmacology

Masato Tamura¹  Susumu Kagawa¹  Yoshihiro Tsuruo²  Kazunori Ishimura²  Kyoji Morita³ 

[1] Department of Urology, Tokushima University School of Medicine;Department of Anatomy, Tokushima University School of Medicine;Department of Pharmacology, Tokushima University School of Medicine

关键词: NADPH diaphorase;    Flavonoid;    Quercetin;   

DOI  :  10.1254/jjp.65.371

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(7)The effects of flavonoids on NADPH diaphorase activity were studied in vitro, and we found that the enzyme activity was markedly inhibited by quercetin. This inhibitory action was shown to be accompanied by an increase in the apparent Km value of the enzyme for the cofactor NADPH, with a decrease in the Vmax, and an increase in the apparent Km for the substrate nitro blue tetrazolium, without any significant change in the Vmax. These results indicate that quercetin may directly inhibit NADPH diaphorase, thus suggesting the possibility that this compound may be able to inhibit the production of nitric oxide in the brain.

【 授权许可】

Unknown