Dexamethasone Inhibits Nitric Oxide Synthase mRNA Induction by Interleukin-1α and Tumor Necrosis Factor-α in Vascular Smooth Muscle Cells

The Japanese Journal of Pharmacology

Hiroko Adachi¹  Masaaki Miyata³  Toshio Nakaki³  Takao Saruta²  Ryuichi Kato³  Takeshi Marumo³  Hiromichi Suzuki²  Kiyoshi Nagata³  Hiroyasu Esumi¹ 

[1] Biochemistry Division, National Cancer Center Research Institute;Departments of Internal Medicine, Keio University School of Medicine;Departments of Pharmacology, Keio University School of Medicine

关键词: Nitric oxide synthase mRNA;    Interleukin1α;    Tumor necrosis factor-α;    Dexamethasone;    Lipopolysaccharide;   

DOI  :  10.1254/jjp.63.361

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(28)Cited-By(6)ABSTRACT-The effects of interleukin-1α, tumor necrosis factor-α and dexamethasone on the induction of nitric oxide synthase mRNA in rat aortic smooth muscle cells were studied. Neither interleukin-1α (up to 100 U/ml) nor tumor necrosis factor-α (up to 5000 U/ml) was capable of inducing nitrite/nitrate production and nitric oxide synthase mRNA in smooth muscle cells. In contrast, treatment for 12 hr or longer with a combination of the two synergistically induced nitrite/nitrate and cyclic GMP production in cell culture media and nitric oxide synthase mRNA, both of which were prevented by dexamethasone. Contamination with bacterial lipopolysaccharide, which may affect the induction of nitric oxide synthase, was below 30 pg/ml in all experiments. Our findings show that dexamethasone and these cytokines regulate the induction of nitric oxide synthase at the mRNA level in vascular smooth muscle cells.

【 授权许可】

Unknown