Effects of D-Ala2-D-Leu5-Enkephalin, Microinj ected into the Supraoptic and Paraventricular Nuclei, on Urine Outflow Rate

The Japanese Journal of Pharmacology

Tomohiro Matsuda¹  Mayumi Mori¹  Hiromi Tsushima¹ 

[1] Department of Pharmacology, Nagoya City University Medical School

关键词: D-Ala2-D-Leu5-Enkephalin;    Vasopressin;    Antidiuretic effect;    Supraoptic nucleus;    Paraventricular nucleus;   

DOI  :  10.1254/jjp.63.181

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(35)Cited-By(10)The effects of D-Ala2-D-Leu5-enkephalin (DADL, a δ-opioid agonist), microinjected directly into the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei, on urine outflow rate, urinary osmotic pressure, blood pressure, heart rate, respiratory rate and rectal temperature were investigated in water-loaded and ethanol-anesthetized rats. The microinjection of DADL into both the nuclei decreased urine outflow rate in a dose-dependent manner with an increase in urinary osmotic pressure, but did not change the other recorded parameters. The DADL-induced antidiuretic effect in the SON was inhibited by naloxone, but not by atropine, phenoxybenzamine, timolol nor a vasopressin antagonist, d(CH2)5-D-Tyr(Et)VAVP. The effect in the PVN was inhibited by naloxone, atropine, timolol and d(CH2)5-D-Tyr(Et)VAVP, but not by phenoxybenzamine. These results suggest that DADL causes antidiuretic effects mediated through opioid receptors in both the SON and PVN, and the underlying mechanisms are different between them. Involvement of δ-opioid receptors in the DADL-induced antidiureses was discussed.

【 授权许可】

Unknown