Sustained Changes in Acetylcholine and Amino Acid Contents of Brain Regions Following Microsphere Embolism in Rats

The Japanese Journal of Pharmacology

Satoshi Takeo²  Manami Okada²  Taku Taguchi²  Keiko Miyake²  Norio Takagi²  Kouichi Tanonaka²  Kannosuke Fujimori¹ 

[1] Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences;Department of Pharmacology, Tokyo College of Pharmacy

关键词: Acetylcholine;    Amino acid;    Choline;    Ischemia (brain);    Microsphere-embolism;   

DOI  :  10.1254/jjp.62.269

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(49)Cited-By(16)The present study was undertaken to explore changes in neurotransmitters and neuromodulators of brain regions impaired by microsphere embolism-induced, sustained ischemia. Nine hundred microspheres (48 μm) were injected into the right internal carotid artery of rats, and the time course of changes in the triphenyltetrazolium chloride (TTC)-stained areas of their brain slices and acetylcholine and amino acid contents in the cerebral cortex, striatum and hippocampus of both hemispheres were determined. The TTC-unstained area, a measure of infarction, was developed in the right hemisphere by the 3rd day after the embolism, which was similar to that on the 28th day. A marked decline in acetylcholine content of these three regions of the right hemisphere was detected throughout the experiment (28 days). The glutamate, aspartate, GABA, and taurine levels were markedly decreased following microsphere-embolism. Most of these decreases were significantly attenuated during the first 5 days following the embolism, and they then partially recovered with time after the operation. Minor metabolic changes were observed in the left hemisphere. The results suggest that microsphere-embolism induces cerebral infarction and/or sustained damage to acetylcholine and neurotransmitter amino acid synthesis and/or catabolism of the brain regions. This model may provide information concerning the pathophysiological alterations in long-term cerebral ischemia and infarction.

【 授权许可】

Unknown