Enhanced Production of Platelet-Activating Factor in Stimulated Rat Leukocytes Caused by the Blockade of Lysophospholipid Acylation

The Japanese Journal of Pharmacology

Hiroaki Naraba¹  Sachiko Oh-ishi¹  Yohsuke Imai¹  Masahiko Hayashi² 

[1] Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University;Kitasato Institute

关键词: Platelet-activating factor;    Lyso-platelet-activating factor;    Zymosan (opsonized);    Merthiolate;    Triacsin C;   

DOI  :  10.1254/jjp.61.109

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(20)Cited-By(6)We have previously reported that triacsin C, an acyl-CoA synthetase inhibitor, enhanced the production of platelet-activating factor (PAF)in calcium ionophore-activated rat polymorphonuclear leukocytes (PMNs). In this report, we further demonstrated that the production of PAF by PMNs in response to opsonized zymosan was significantly enhanced by pretreatment with triacsin C and also by the preteatment with merthiolate, which was reported to be an inhibitor of acyl-CoA/lysolecithin acyltransferase. Pretreatment with triacsin C or merthiolate also enhanced the lyso-PAF content in the stimulated PMNs. Addition of lyso-PAF in the incubation mixture of PMNs in the presence of opsonized zymosan augumented the production of PAF. The enhancement of PAF production by lyso-PAF has been reported by several authors, and the importance of lyso-PAF in the remodeling pathway of PAF synthesis has been generally recognized. Therefore, from the above findings, it is assumed that blockades of the reacylation of lyso-phospholipids, by inhibitors such as triacsin C and merthiolate, might lead to accumulation of lyso-PAF and might result in the enhancement of PAF production when the remodeling pathway is active.

【 授权许可】

Unknown