Further Studies on the Endogenous Serotonin-Uptake-Inhibitor-Like Substances in the Human Cerebrospinal Fluid

The Japanese Journal of Pharmacology

Shinichiro Goto¹  Yasumitsu Yamanaka¹  Toru Egashira¹ 

[1] Department of Pharmacology, Oita Medical University

关键词: Serotonin uptake;    Paroxetine binding;    Cerebrospinal fluid (human);    Brain (monkey);    Antidepressant (endogenous);   

DOI  :  10.1254/jjp.61.51

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(31)Cited-By(1)The properties of endogenous substances that inhibit 5HT uptake in human cerebrospinal fluid (CSF)were investigated. Human CSF was loaded onto a Sephadex G-25 column, and each fraction was tested for its ability to inhibit [3H]paroxetine binding in monkey brain preparations. We found four different inhibitory peaks with respective molecular weights (M.W.)of > 12400, 2000 and two of 3H]paroxetine binding noncompetitively (decreased Bmax and did not change Kd)and inhibited [3H]5HT uptake noncompetitively (decreased Vmax and did not change Km), but had no effect on either [3H]norepinephrine uptake or [3H]dopamine uptake. These results suggest that the endogenous substances that selectively inhibit 5HT uptake are present in human CSF as low molecular weight compounds.

【 授权许可】

Unknown