Flow Cytometric Estimation of the Effect of Ginkgo Biloba Extract on the Content of Hydrogen Peroxide in Dissociated Mammalian Brain Neurons

The Japanese Journal of Pharmacology

Akemi Hayashi³  Toshiko Ueha³  Lumi Chikahisa¹  Katsuhiko Noda²  Yasuo Oyama³ 

[1] Taiho Pharmaceutical Co., Ltd.;Food Science, Faculty of Integrated Arts and Sciences, The University of Tokushima;Laboratories for Pharmacology, The University of Tokushima

关键词: Ginkgo biloba extract;    Brain neuron;    Hydrogen peroxide;   

DOI  :  10.1254/jjp.60.385

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(30)The effect of Ginkgo biloba extract (GBE) on the content of hydrogen peroxide was estimated in cerebellar neurons dissociated from rats, by means of a flow-cytometer and 2'', 7''-dichloro-fluorescin (DCF) diacetate, a fluorescent dye for intracellular hydrogen peroxide. The GBE started to reduce the DCF fluorescence of the neuron at 0.1 μg/ml to 0.3 μg/ml. Further increases in the GBE concentration (up to 3 μg/ml) produced a dose-dependent decrease in the DCF fluorescence, suggesting that GBE reduces the content of hydrogen peroxide or suppresses the reactive oxygen species (ROS) formation of cerebellar neurons. The present technique may be useful for preliminary evaluations of agents affecting the ROS formation in mammalian brain neurons.

【 授权许可】

Unknown