In Vivo Release of Dopamine by Perfusion of 1-Methyl-4-Phenylpyridinium Ion in the Striatum with a Microdialysis Technique

The Japanese Journal of Pharmacology

Toshio Obata²  Chuang C. Chiueh¹  Yasumitsu Yamanaka² 

[1] National Institute of Mental Health;Department of Pharmacology, Oita Medical University

关键词: 1-Methyl-4-phenylpyridinium ion (MPP+);    Monoamine oxidase (B-form);    Parkinsonism;   

DOI  :  10.1254/jjp.60.311

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(13)Cited-By(18)We examined the effects of 1-methyl-4-phenylpyridinium ion (MPP+) on the release of DA in rat striatum by the in vivo microdialysis technique. For this study, we made a suitable microdialysis probe from a 22-G needle, microliter pipette tip, silica tube and polyethylene tube. Such a repairable microdialysis probe can be easily made from readily available and inexpensive materials. DA release, as determined by the 3-methoxytyramine level, was dose-dependently increased by MPP+ (1-10 mM). Only the presence of a 1 mM concentration of MPP+ in the dialysate significantly decreased the level of the DA metabolite DOPAC, while administration of higher MPP+ concentrations resulted in no significant change.

【 授权许可】

Unknown