A single nucleotide polymorphism possibly associated with fat deposition is methylated in the bovine TFAM promoter

Acta agriculturae Slovenica

Jennifer J. MICHAL²  Tanja KUNEJ³  Nancy S. MAGNUSON¹  Peter DOVČ³  Zhihua JIANG²  Zeping WANG¹ 

[1]Washington State University, School of Molecular Biosciences

[2]Washington State University, Department of Animal Sciences

[3]University of Ljubljana, Biotechnical Faculty, Department of Animal Science

关键词: cattle;    genetics;    genetic polymorphism;    candidate genes;    gene promoters;    TFAM;    DNA methylation;    CpG island;    fat deposition;   

DOI  :  10.14720/aas.2016.108.2.1

学科分类：农业科学（综合）

来源: Univerza v Ljubljani * Biotehniska Fakulteta / University of Ljubljana * Biotechnical Faculty

【 摘 要 】

Mitochondrial transcription factor A ( TFAM ) is a nuclear-encoded mitochondrial protein that plays an important role in energy metabolism and is a candidate gene for fat deposition in cattle and human. In the present study, we characterized the methylation status of bovine TFAM promoter flanking the single nucleotide polymorphism (SNP) rs42159487C>T, previously reported to affect fat deposition. Our results showed that the cytosine at this SNP position is methylated and therefore results in gain/loss of the functional CpG locus (5 m C>T). Promoter constructs developed based on three TFAM SNPs showed that the C/C/C haplotype associated with fat deposition in beef cattle had lower promoter function/activity than A/T/T haplotype. Our results imply that genetic variability underlying gain/loss of the CpG sites in the nuclear-encoded mitochondrial proteins might be good candidate loci for fat deposition phenotypes.

【 授权许可】

Unknown