High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb

Acta agriculturae Slovenica

Ahmad Farhad Talebi²  Ghorbanali Nematzadeh¹  Sara Kabirnataj¹  Jafar Zolala³ 

[1] Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University;Department of Genetics, Faculty of Microbial Biotechnology, Semnan University;Department of Plant Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman

关键词: Chicory;    A. rhizogenes;    hairy root;    GUS;    A13;   

DOI  :  10.14720/aas.2016.107.2.06

学科分类：农业科学（综合）

来源: Univerza v Ljubljani * Biotehniska Fakulteta / University of Ljubljana * Biotechnical Faculty

【 摘 要 】

Plant roots can be manipulated by Agrobacterium rhizogenes to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory ( Cichorium intybus L.) using A. rhizogenes . The maximum chicory hairy roots induction was achieved using A13 strain. The observation confirmed that MS medium was more effective on hairy root growth. Dried biomass accumulation of hairy roots infected by A13 strain was 1.10 g l -1 in MS medium which was significantly higher than those grown in LS and B5 medium (0.88 and 0.72 g l -1 , respectively). Beta-glucuronidase (GUS) gene was introduced by A13 strain carrying the pCAMBIA1304 binary vector. The results showed that the highest frequency of transformation (63.15 %) was achieved using A13 strain and MS cultivation medium. Detection of GUS and hpt II genes by PCR and GUS histochemical localization confirmed the integrative transformation in hairy roots. In conclusion, the whole process was successfully optimized as a pre-step to manipulate the chicory hairy root cells to improve the unique potential of secondary metabolite production.

【 授权许可】

Unknown