The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits

Acta agriculturae Slovenica

Dunja BANDELJ²  Matjaž HLADNIK²  Irma VUK²  Jernej JAKŠE¹ 

[1]University of Ljubljana, Centre for Plant Biotechnology, Jamnikarjeva 101, SI - 1000 Ljubljana, Slovenia

[2]University of Primorska, Science and Research Centre, Garibaldijeva 1, SI - 6000 Koper, Slovenia

关键词: vitis vinifera;    grapevines;    genetic variation;    land varieties;    plant anatomy;    germplasm;    genetic markers;    selection;   

DOI  :  10.14720/aas.2014.103.1.06

学科分类：农业科学（综合）

来源: Univerza v Ljubljani * Biotehniska Fakulteta / University of Ljubljana * Biotechnical Faculty

【 摘 要 】

The genetic diversity and ampelographic variability of autochthonous red wine cultivar ‘Refošk’ ( Vitis vinifera L.) grown in Slovenia were evaluated with AFLP molecular markers and OIV descriptors, respectively. SSR molecular markers were employed to confirm cultivar identity of analysed samples. Eight AFLP primer combinations, one was monomorphic, produced 16 polymorphic markers in 41 out of 113 samples, what classified samples into monomorphic and polymorphic group. Dendrogram constructed with simple matching coefficient and unweighted pair-group method analysis presented genetic diversity within polymorphic group. Refošk biotypes from monomorphic and polymorphic groups were evaluated with 22 OIV descriptors related to bunch, berry and must, but on the basis of ampelographic characterization samples were not differentiated among two major groups obtained with AFLP analysis. Results of genetic analysis indicated that ‘Refošk’ originated from closely related plants that are phenotypically very similar. With regard to low observed genetic diversity more attention should be dedicated to the selection in order to conserve remaining genetic diversity.

【 授权许可】

Unknown