TFE3 Is a bHLH-ZIP-type Transcription Factor that Regulates the Mammalian Golgi Stress Response

Cell Structure and Function

Hiroshi Kitagawa¹  Sadao Wakabayashi²  Masaya Oku³  Naoko Araki²  Yumeto Kawai²  Sachiko Midori²  Soichiro Tanakura²  Shogo Sawaguchi²  Satomi Nadanaka¹  Yui Shimada²  Mai Taniguchi²  Mayu Yamamoto²  Shogo Yamaguchi²  Hiderou Yoshida²  Natsumi Fujita²  Yuki Nakamura²  Yasuyo Matsumura² 

[1] Department of Biochemistry, Kobe Pharmaceutical University;Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo;Department of Biophysics, Graduate School of Science, Kyoto University

关键词: Golgi stress;    proteoglycan;    sialyltransferase;    glycosylation;    ER stress;   

DOI  :  10.1247/csf.14015

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(76)Cited-By(9)Supplementary materials(7)The Golgi stress response is a mechanism by which, under conditions of insufficient Golgi function (Golgi stress), the transcription of Golgi-related genes is upregulated through an enhancer, the Golgi apparatus stress response element (GASE), in order to maintain homeostasis in the Golgi. The molecular mechanisms associated with GASE remain to be clarified. Here, we identified TFE3 as a GASE-binding transcription factor. TFE3 was phosphorylated and retained in the cytoplasm in normal growth conditions, whereas it was dephosphorylated, translocated to the nucleus and activated Golgi-related genes through GASE under conditions of Golgi stress, e.g. in response to inhibition of oligosaccharide processing in the Golgi apparatus. From these observations, we concluded that the TFE3-GASE pathway is one of the regulatory pathways of the mammalian Golgi stress response, which regulates the expression of glycosylation-related proteins in response to insufficiency of glycosylation in the Golgi apparatus.

【 授权许可】

Unknown