A Biphenyl Type Two-Photon Fluorescence Probe for Monitoring the Mitochondrial Membrane Potential

Cell Structure and Function

Seiji Tani¹  Yuki Kojima³  Jun Kawamata²  Masataka Kinjo⁵  Hazuki Kinoshita⁵  Shintaro Mikuni⁵  Yasutaka Suzuki²  Kengo Yamada¹  Akira Sasaki⁴  Hiroki Moritomo² 

[1] Graduate School of Science and Engineering, Yamaguchi University;Graduate School of Medicine, Yamaguchi University;Department of Chemistry, Faculty of Science, Yamaguchi University;Laboratory for Nano-Bio Probes, Quantitative Biology Center;Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University

关键词: Two-photon fluorescence;    fluorescence probe;    mitochondrial membrane potential;    biphenyl derivative;   

DOI  :  10.1247/csf.14006

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-‍dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitochondrial membrane potential was high. As the mitochondrial membrane potential decreased following the addition of carbonyl cyanide m-chlorophenyl hydrazine, BP6 moved from the mitochondria to the nucleus in a reversible manner, depending on the mitochondrial membrane potential status. The maximum value of the two-photon absorption cross-section of BP6 is 250 GM (1 GM=1×10–50 cm4 s molecules–1 photon–1). This value is 3 and 30 times larger, respectively, than that of the conventional mitochondria selective probes, rhodamine 123 and green fluorescence protein. These results suggest that BP6 should be useful for monitoring mitochondrial membrane potential by two-photon excitation.

【 授权许可】

Unknown