In vitro Assembly Properties of Human Type I and II Hair Keratins

Cell Structure and Function

Yuki Arakawa¹  Yuko Honda³  Sadahiko Masuko³  Kenzo Koike²  Shoji Ando¹  Yuki Kubo⁴ 

[1] Faculty of Biotechnology and Life Science, Sojo University;Beauty Research Center, KAO Corporation;Department of Anatomy and Physiology, Faculty of Medicine, Saga University;Department of Biomolecular Sciences, Faculty of Medicine, Saga University

关键词: cytoskeleton;    hair keratins;    intermediate filaments;    protein-protein interaction;    self-assembly;   

DOI  :  10.1247/csf.13021

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(46)Multiple type I and II hair keratins are expressed in hair-forming cells but the role of each protein in hair fiber formation remains obscure. In this study, recombinant proteins of human type I hair keratins (K35, K36 and K38) and type II hair keratins (K81 and K85) were prepared using bacterial expression systems. The heterotypic subunit interactions between the type I and II hair keratins were characterized using two-dimensional gel electrophoresis and surface plasmon resonance (SPR). Gel electrophoresis showed that the heterotypic complex-forming urea concentrations differ depending on the combination of keratins. K35-K85 and K36-K81 formed relatively stable heterotypic complexes. SPR revealed that soluble K35 bound to immobilized K85 with a higher affinity than to immobilized K81. The in vitro intermediate filament (IF) assembly of the hair keratins was explored by negative-staining electron microscopy. While K35-K81, K36-K81 and K35-K36-K81 formed IFs, K35-K85 afforded tight bundles of short IFs and large paracrystalline assemblies, and K36-K85 formed IF tangles. K85 promotes lateral association rather than elongation of short IFs. The in vitro assembly properties of hair keratins depended on the combination of type I and II hair keratins. Our data suggest the functional significance of K35-K85 and K36-K81 with distinct assembly properties in the formation of macrofibrils.

【 授权许可】

Unknown