Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes

Cell Structure and Function

Hye-Won Shin¹  Tomoko Ueda¹  Satoshi Waguri²  Takashi Murayama³  Hiroyuki Takatsu¹  Yohei Katoh¹  Kazuhisa Nakayama¹  Senye Takahashi¹ 

[1] Graduate School of Pharmaceutical Sciences, Kyoto University;Department of Anatomy and Histology, Fukushima Medical University School of Medicine;Department of Pharmacology, Juntendo University School of Medicine

关键词: recycling endosome;    transferrin receptor;    spindle pole;    cytokinesis;    dynein;   

DOI  :  10.1247/csf.12028

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(40)Cited-By(6)Supplementary materials(10)Upon cell division, not only cells themselves but also their organelles undergo drastic shape changes, although the behaviors of organelles other than the Golgi apparatus remain poorly understood. We followed the spatiotemporal changes in the localization of transferrin receptor (TfnR) and other proteins. In early mitotic phases, a population of proteins cycling through the endocytic recycling compartment (ERC) exhibits a distinct spatiotemporal change from that of Golgi proteins. In prophase/prometaphase, when the cell surface-to-volume ratio is reaching its minimum, the ERC proteins are transiently assembled around the centrated centrosome in a microtubule- and dynein-dependent manner, and soon separated polewards into two clusters concomitant with separation of duplicated centrosomes. Electron microscopic analysis revealed that endosomal vesicles containing endocytosed transferrin cluster tightly around centrosomes without fusing with one another. As cytokinesis proceeds, the clusters gradually collapse, and the ERC proteins reassemble around the furrowing equatorial region. FRAP (fluorescence recovery after photobleaching) analyses of EGFP-TfnR-expressing cells revealed minimal membrane exchange between the endosomal clusters and other cellular compartments until anaphase/telophase, when membrane traffic resumes. Our observations indicate that ERC clustering around centrosomes plays a fundamental role in restricting membrane delivery to the plasma membrane during early mitotic phases, when the cell surface-to-volume ratio reaches its minimum.

【 授权许可】

Unknown