Novel Cis-acting Element GASE Regulates Transcriptional Induction by the Golgi Stress Response

Cell Structure and Function

Mai Taniguchi²  Sadao Wakabayashi²  Aya Uemura³  Hiderou Yoshida³  Masaya Oku³  Soichiro Tanakura²  Yoshio Misumi⁴  Miwa Sohda¹ 

[1] Division of Oral Biochemistry, Graduate School of Medical and Dental Sciences, Niigata University;Department of Biochemistry and Molecular Biology, Graduate School of Life Science, University of Hyogo;Department of Biophysics, Graduate School of Science, Kyoto University;Department of Biochemistry, School of Medicine, Fukuoka University

关键词: Golgi apparatus;    Golgi stress response;    ER stress response;    unfolded protein response;   

DOI  :  10.1247/csf.10014

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(45)Cited-By(18)Supplementary materials(1)When increased production of secretory proteins overwhelms the capacity of the endoplasmic reticulum (ER) and the Golgi apparatus, eukaryotic cells expand their capacity to sustain secretory function. The capacity of the ER is enhanced by the mechanism called the ER stress response, but the mechanism regulating Golgi capacity (the Golgi stress response) has remained unclear. Here, we found that transcription of Golgi-related genes, including glycosylation enzymes as well as factors involved in post-Golgi vesicular transport and maintenance of Golgi structure, was upregulated upon treatment with monensin, an ionophore that disrupts the function of acidic organelles, including the Golgi apparatus and lysosomes by neutralizing their lumen. This transcriptional induction was found to be commonly regulated by a novel cis-acting element called the Golgi apparatus stress response element (GASE), whose consensus sequence is ACGTGgc. When the function of the Golgi apparatus was specifically disturbed by overexpression of GCP60, a Golgi-localized protein that binds to giantin, transcription from GASE was significantly induced. These results suggest that mammalian cells have the Golgi stress response, and that GASE regulates transcriptional induction involved in the Golgi stress response.

【 授权许可】

Unknown