Increased Motility and Invasiveness in Tumor Cells That Survive 10 Gy Irradiation

Cell Structure and Function

Rie Yamazaki⁵  Seiichiro Ishihara¹  Hirotaka Nakamura⁵  Takeshi Nishioka⁵  Kaori Tsutsumi⁵  Yusuke Ohba³  Natsuka Yazawa⁵  Hiroki Shirato²  Hideaki Kawaguchi³  Hisashi Haga¹  Motoaki Yasuda⁴  Masumi Tsuda³ 

[1] Division of Biological Sciences, Graduate School of Science, Hokkaido University;Department of Radiology, Hokkaido University Graduate School of Medicine;Laboratory of Pathophysiology and Signal Transduction, Hokkaido University Graduate School of Medicine;Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University;Department of Biomedical Science and Engineering, Faculty of Health Sciences, Hokkaido University

关键词: non-small cell lung cancer;    radiotherapy;    MMP;    invasion;    cell motility;   

DOI  :  10.1247/csf.09006

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(22)Cited-By(10)Radiotherapy is an important noninvasive treatment for many types of cancer. However, it has been reported that the proliferative, invasive, and metastatic capacities of tumor cells can be increased in the repopulated tumors that survive radiotherapy. We have previously established a radiation-surviving cell model for the human non-small cell lung cancer cell line H1299 by harvesting relic cells 14 days after irradiation (IR cells). Here, we report that cell invasion, cell migration, and cell adhesion are enhanced in these surviving cancer cells. The mRNA expression levels of matrix metalloproteinases (MMPs), including mmp1, mmp2, and mmp9, were upregulated in IR cells compared with parental cells. A gelatin zymogram, wound healing assay, and invasion assay showed increased MMP activity, cell motility, and invasiveness in IR cells, respectively. Moreover, IR cells adhered more tightly to collagen-coated dishes than parental cells. Consistently, paxillin, phosphorylated FAK, integrin β1, and vinculin were strongly localized at focal adhesions in IR cells, as visualized by immunofluorescence. In this report, we identify molecules responsible for the malignant properties of tumor cells that survive irradiation. These molecules may be important therapeutic targets for the control of repopulated tumors after radiotherapy.

【 授权许可】

Unknown