A Mercury Arc Lamp-Based Multi-Color Confocal Real Time Imaging System for Cellular Structure and Function

Cell Structure and Function

Takeharu Nagai¹  Kenta Saito¹  Kentaro Kobayashi¹  Tomomi Tani² 

[1] Nikon imaging center, Research Institute for Electronic Science, Hokkaido University;Laboratory for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University

关键词: confocal microscopy;    live imaging;    laser;    arc lamp;    Nipkow disk;   

DOI  :  10.1247/csf.08015

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(21)Cited-By(3)Supplementary materials(2)Multi-point scanning confocal microscopy using a Nipkow disk enables the acquisition of fluorescent images with high spatial and temporal resolutions. Like other single-point scanning confocal systems that use Galvano meter mirrors, a commercially available Nipkow spinning disk confocal unit, Yokogawa CSU10, requires lasers as the excitation light source. The choice of fluorescent dyes is strongly restricted, however, because only a limited number of laser lines can be introduced into a single confocal system. To overcome this problem, we developed an illumination system in which light from a mercury arc lamp is scrambled to make homogeneous light by passing it through a multi-mode optical fiber. This illumination system provides incoherent light with continuous wavelengths, enabling the observation of a wide range of fluorophores. Using this optical system, we demonstrate both the high-speed imaging (up to 100 Hz) of intracellular Ca2+ propagation, and the multi-color imaging of Ca2+ and PKC-γ dynamics in living cells.

【 授权许可】

Unknown