The Organization of Histone H3 Modifications as Revealed by a Panel of Specific Monoclonal Antibodies

Cell Structure and Function

Nanako Takizawa¹  Yoko Hayashi-Takanaka¹  Yuji Goto¹  Hiroshi Kimura¹  Naohito Nozaki² 

[1] Nuclear Function and Dynamics Unit, HMRO, Graduate School of Medicine, Kyoto University;Kanagawa Dental College

关键词: chromatin;    epigenetics;    histone modification;    monoclonal antibody;    senescence;   

DOI  :  10.1247/csf.07035

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(29)Cited-By(89)Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifically recognize different modifications on K4, K9, and K27 residues on histone H3. By using these antibodies for chromatin immunoprecipitation and immunoblotting, we analyzed the relationship between different modifications in nearby nucleosomes in human cells. Within a few nucleosome neighbors, trimethyl-K4 was associated with acetyl-K27, rather than with dimethyl-K4 and acetyl-K9, consistent with their co-localization on active promoters. Furthermore, simultaneous immunofluorescence using directly-labeled antibodies revealed that di- and tri-methylation on K4 was diminished during replicative senescence. These highly-reliable and fully-characterized monoclonal antibodies may facilitate future epigenomic studies on healthy and diseased cells.

【 授权许可】

Unknown