Endothelial Cells Constituting Blood-nerve Barrier Have Highly Specialized Characteristics as Barrier-forming Cells

Cell Structure and Function

Toshihiko Maeda³  Fumitaka Shimizu³  Sumio Ohtsuki²  Masaaki Abe³  Masuo Obinata¹  Masatsugu Ueda⁴  Yasuteru Sano³  Hiroto Nakayama³  Ri-ichi Takahashi⁴  Takashi Kanda³  Tetsuya Terasaki² 

[1] Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University;Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University;Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine;The YS Institute, Inc.

关键词: blood-nerve barrier;    peripheral nerve microvascular endothelial cells;    tight junction;    claudin-5;    transporter;   

DOI  :  10.1247/csf.07015

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(42)Cited-By(24)In autoimmune disorders of the peripheral nervous system (PNS) such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered as a key step in the disease process. Hence, it is important to know the cellular property of peripheral nerve microvascular endothelial cells (PnMECs) constituting the bulk of BNB. Although many in vitro models of the blood-brain barrier (BBB) have been established, very few in vitro BNB models have been reported so far. We isolated PnMECs from transgenic rats harboring the temperature-sensitive SV40 large T-antigen gene (tsA58 rat) and investigated the properties of these “barrier-forming cells”. Isolated PnMECs (TR-BNBs) showed high transendothelial electrical resistance and expressed tight junction components and various types of influx as well as efflux transporters that have been reported to function at BBB. Furthermore, we confirmed the in vivo expression of various BBB-forming endothelial cell markers in the endoneurium of a rat sciatic nerve. These results suggest that PnMECs constituting the bulk of BNB have a highly specialized characteristic resembling the endothelial cells forming BBB.

【 授权许可】

Unknown