1,25-Dihydroxyvitamin D3 Suppresses Gene Expression of Eukaryotic Translation Initiation Factor 2 in Human Promyelocytic Leukemia HL-60 Cells

Cell Structure and Function

Hiroyasu Ichikawa²  Kouichi Abe²  Ryoko Kuruto-Niwa¹  Mamoru Isemura²  Sumio Hayakawa²  Koji Tsushima²  Ryushi Nozawa¹  Takuji Suzuki²  Yu Koyama² 

[1] Microbiology and Host Defense, School of Food and Nutritional Sciences, University of Shizuoka;Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21st Century, University of Shizuoka

关键词: vitamin D3;    differentiation;    gene expression;    initiation factor;    HL-60;   

DOI  :  10.1247/csf.30.1

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(23)Cited-By(8)The physiologically active metabolite of vitamin D3, 1α,25-dihydroxyvitamin D3 (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.

【 授权许可】

Unknown