Systematic Analysis of SNARE Molecules in Arabidopsis: Dissection of the post-Golgi Network in Plant Cells

Cell Structure and Function

Masa H. Sato²  Ryosuke L. Ohniwa¹  Takashi Ueda³  Akihiko Nakano³  Kunio Takeyasu¹  Tomohiro Uemura¹ 

[1] Graduate School of Biostudies, Kyoto University;Graduate School of Human and Environmental Studies, Kyoto University;Department of Biological Sciences, Graduate School of Science, University of Tokyo

关键词: Arabidopsis;    membrane traffic;    vesicle fusion;    SNARE;   

DOI  :  10.1247/csf.29.49

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(71)Cited-By(200)In all eucaryotic cells, specific vesicle fusion during vesicular transport is mediated by membrane-associated proteins called SNAREs (soluble N-ethyl-maleimide sensitive factor attachment protein receptors). Sequence analysis identified a total of 54 SNARE genes (18 Qa-SNAREs/Syntaxins, 11 Qb-SNAREs, 8 Qc-SNAREs, 14 R-SNAREs/VAMPs and 3 SNAP-25) in the Arabidopsis genome. Almost all of them were ubiquitously expressed through out all tissues examined. A series of transient expression assays using green fluorescent protein (GFP) fused proteins revealed that most of the SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar compartment (PVC) and vacuoles, 2 in TGN/PVC/vacuoles, and 1 in TGN/PVC/plasma membrane. Some SNARE proteins showed multiple localization patterns in two or more different organelles, suggesting that these SNAREs shuttle between the organelles. Furthermore, the SYP41/SYP61-residing compartment, which was defined as the TGN, was not always located along with the Golgi apparatus, suggesting that this compartment is an independent organelle distinct from the Golgi apparatus. We propose possible combinations of SNARE proteins on all subcellular compartments, and suggest the complexity of the post-Golgi membrane traffic in higher plant cells.

【 授权许可】

Unknown