Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene

Cell Structure and Function

Yoshiaki Tabuchi³  Masuo Obinata¹  Masatsugu Ueda²  Ri-ichi Takahashi² 

[1] Institute of Development, Aging and Cancer, Tohoku University;YS New Technology Institute Inc.;Life Science Research Center, Toyama Medical and Pharmaceutical University

关键词: temperature-sensitive simian virus 40 large T-antigen;    transgenic rat;    Sertoli cell;    sulfated glycoprotein-2;    transferrin;    steel factor;   

DOI  :  10.1247/csf.28.87

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(41)Cited-By(8)Transgenic mice and rats harboring temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene are useful for establishing cell lines from tissues. We succeeded in establishing a conditionally immortalized testicular Sertoli cell line, RT3-3, from adult transgenic rats harboring the oncogene. The cells grew at permissive (33°C) and intermediate (37°C) temperatures but not at nonpermissive temperature (39°C). Large T-antigen was expressed at 33 and 37°C, whereas the expression level was gradually decreased at 39°C, suggesting that the temperature-sensitive growth characteristics arise as a result of the function of tsSV40 large T-antigen. The cells showed biochemical features associate with normal Sertoli cells including expressions of mRNAs of sulfated glycoprotein-2 (SGP-2), transferrin (TF) and steel factor. Quantitative polymerase chain reaction revealed that nonpermissive temperature induced increase in the level of SGP-2. Moreover, levels of SGP-2 and/or TF were significantly elevated in the cells treatment with sodium butyrate and retinoic acid, inducers of cellular differentiation. To our knowledge, this is the first report of the establishment of a testicular Sertoli cell line from the transgenic rats. Thus, the conditionally immortalized cell line RTS3-3 with unique characteristics may serve as good experimental in vitro models for basic and applied biology of testicular Sertoli cells.

【 授权许可】

Unknown