Endocytic Pathway in Mouse Cardiac Cells

Cell Structure and Function

Denise da Gama Jaen Batista²  Renata Alves Mota¹  Maria de Nazareth Leal Meirelles¹  Maria de Nazaré Correia Soeiro² 

[1] Lab. Ultra-estrutura Celular, Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ;Lab. Biologia Celular

关键词: cardiomyocytes;    endocytosis;    electron microscopy;    Trypanosoma cruzi;   

DOI  :  10.1247/csf.27.469

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(43)Cited-By(1)Primary cultures of heart muscle cells provide powerful tools for cardiac cell biological research that permits both physiological and biochemical approaches. In the present study we analyzed the endocytosis of cardiac cells and presented morphological characterization of the endocytic machinery using markers, which enabled us to follow the fluid-phase, receptor-mediated endocytosis and the internalization of large particles. Our results demonstrated the route of the internalized cargo to early endosomes followed or not by its discharge in the late compartments. We also confirmed the ability of cardiac muscle cells to ingest large particles such as the mannosylated ligand zymosan A, and even internalize whole eukaryotic cells such as the protozoan parasite Trypanosoma cruzi. Since endocytosis is involved in many important cellular functions, the present work contributes to the knowledge of possible additional roles played by cardiac muscle cells besides their well known ability to act as physically energetic cells in the body, constantly contracting without tiring.

【 授权许可】

Unknown