Gene Expression of Type I and Type III Collagen by Mechanical Stretch in Anterior Cruciate Ligament Cells

Cell Structure and Function

Hisashi Kurosawa²  Yoriko Atomi³  Toshihiro Akaike¹  Tadashi Sasagaw¹  Sung-Gon Kim² 

[1] 2Department of Biomolecular Engineering, Tokyo Institute of Technology;Department of Orthopaedic Surgery, Juntendo University;3Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

关键词: anterior cruciate ligament (ACL);    fibroblast;    mechanical stretch;    type I collagen;    type III collagen;    transforming growth factor (TGF)-β1;   

DOI  :  10.1247/csf.27.139

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(31)Cited-By(86)Mechanical stretch affects the healing and remodeling process of the anterior cruciate ligament (ACL) after surgery in important ways. In this study, the effects of mechanical stress on gene expression of type I and III collagen by cultured human ACL cells and roles of transforming growth factor (TGF)-β1 in the regulation of mechanical strain-induced gene expression were investigated. Uniaxial cyclic stretch was applied on ACL cells at 10 cycles/min with 10% length stretch for 24 h. mRNA expression of the type I and type III collagen was increased by the cyclic stretch. TGF-β1 protein in the cell culture supernatant was also increased by the stretch. In the presence of anti-TGF-β1 antibody, stretch-induced increase in type I and type III mRNA expression was markedly ablated. The results suggest that the stretch-induced mRNA expression of the type I and type III collagen is mediated via an autocrine mechanism of TGF-β1 released from ligament cells.

【 授权许可】

Unknown