Possible Involvement of Hic-5, a Focal Adhesion Protein, in the Differentiation of C2C12 Myoblasts

Cell Structure and Function

Motoko Shibanuma¹  Kiyoshi Nose¹  Yasuhiko Iwabuchi¹ 

[1] Department of Microbiology, Showa University School of Pharmaceutical Sciences

关键词: Hic-5;    C2C12;    myogenic differentiation;   

DOI  :  10.1247/csf.27.21

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(23)Cited-By(7)Hic-5, a focal adhesion protein, has been implicated in cellular senescence and differentiation. In this study, we examined its involvement in myogenic differentiation. The hic-5 expression level in growing C2C12 myoblasts increased slightly on the first day and then gradually decreased until no hic-5 was detectable after 7 days of differentiation. In vivo, its expression level declined in the thigh and the calf skeletal muscle of mouse embryos after birth. The introduction of an antisense expression vector of hic-5 into C2C12 cells decreased the number of clones expressing the myosin heavy chain (MHC) upon exposure to the differentiation medium. In the cloned cells with low levels of hic-5, the efficiency of myotube formation was significantly reduced. The expression levels of MyoD, myogenin, MHC and p21 were also reduced in these clones. The results suggested that hic-5 plays a role in the initial stage of myogenic differentiation.

【 授权许可】

Unknown