Proliferation Stage-dependent Expression of DNA Methyltransferase (Dnmt1) in Mouse Small Intestine

Cell Structure and Function

Daisuke Watanabe¹  Shoji Tajima²  Liheng Shi²  Isao Suetake²  Masahiko Nakamura² 

[1] Institute for Molecular and Cellular Biology, Osaka University;Institute for Protein Research, Osaka University

关键词: DNA methyltransferase;    small intestine;    cell proliferation;   

DOI  :  10.1247/csf.26.79

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(33)Cited-By(13)In cultured cells, the maintenance-type DNA methyltransferase (Dnmt1) is highly expressed during the proliferation stage. In the present study, we detected significant expression of Dnmt1 protein in the nuclear fraction of mouse small intestine. From its mobility in SDS polyacrylamide gel electrophoresis and the specific antibodies against the somatic cell-type Dnmt1, Dnmt1 was determined as a somatic cell type. Immunofluorescence study revealed that the Dnmt1 was highly expressed in the proliferating stem cells in crypts, and was localized in the nuclei. The present results indicate that the expression of Dnmt1 in vivo is also under the control of cell proliferation as in cultured cells.

【 授权许可】

Unknown