P2Y-Purinoceptor Mediated Inhibition of L-type Ca2+ Channels in Rat Pancreatic β-Cells

Cell Structure and Function

Mitsuhiro Nakazaki¹  Chuwa Tei¹  Kazuro Yaekura¹  Qi Gong¹  Shingo Morimitsu¹  Masafumi Kakei¹  Nobuyuki Koriyama¹ 

[1] First Department of Internal Medicine, Faculty of Medicine, Kagoshima University

关键词: action potentials;    Ca2+ channels;    cytosolic Ca2+;    extracellular ATP;    pancreatic β-cells;    P2Y purinoceptor;    G-proteins;   

DOI  :  10.1247/csf.25.279

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(54)Cited-By(13)We used the patch-clamp technique to study the effects of extracellular ATP on the activity of ion channels recorded in rat pancreatic β-cells. In cell-attached membrane patches, action currents induced by 8.3 mM glucose were inhibited by 0.1 mM ATP, 0.1 mM ADP or 15 μM ADPβS but not by 0.1 mM AMP or 0.1 mM adenosine. In perforated membrane patches, action potentials were measured in current clamp, induced by 8.3 mM glucose, and were also inhibited by 0.1 mM ATP with a modest hyperpolarization to −43 mV. In whole-cell clamp experiments, ATP dose-dependently decreased the amplitudes of L-type Ca2+ channel currents (ICa) to 56.7±4.0% (p<0.001) of the control, but did not influence ATP-sensitive K+ channel currents observed in the presence of 0.1 mM ATP and 0.1mM ADP in the pipette. Agonists of P2Y purinoceptors, 2-methylthio ATP (0.1 mM) or ADPβS (15 μM) mimicked the inhibitory effect of ATP on ICa, but PPADS (0.1 mM) and suramin (0.2 mM), antagonists of P2 purinoceptors, counteracted this effect. When we used 0.1 mM GTPγS in the pipette solution, ATP irreversibly reduced ICa to 58.4±6.60f the control (p<0.001). In contrast, no inhibitory effect of ATP was observed when 0.2 mM GDPβS was used in the pipette solution. The use of either 20 mM BAPTA instead of 10 mM EGTA, or 0.1 mM compound 48/80, a blocker of phospholipase C (PLC), in the pipette solution abolished the inhibitory effect of ATP on ICa, but 1 μM staurosporine, a blocker of protein kinase C (PKC), did not. When the β-cells were pretreated with 0.4 μM thapsigargin, an inhibitor of the endoplasmic reticulum (ER) Ca2+ pump, ATP lost the inhibitory effect on ICa. These results suggest that extracellular ATP inhibits action potentials by Ca2+-induced ICa inhibition in which an increase in cytosolic Ca2+ released from thapsigargin-sensitive store sites was brought about by a P2Y purinoceptor-coupled G-protein, PI-PLC and IP3 pathway.

【 授权许可】

Unknown