Interaction of Actin Filaments with the Plasma Membrane in Amoeba proteus: Studies Using a Cell Model and Isolated Plasma Membrane

Cell Structure and Function

Tomomi Kawakatsu¹  Asako Kikuchi¹  Seiji Sonobe¹  Teruo Shimmen¹ 

[1] Department of Life Science, Faculty of Science, Himeji Institute of Technology

关键词: actin filaments;    Amoeba proteus;    amoeboid movement;    calcium ion;    plasma membrane;    pseudopod formation;   

DOI  :  10.1247/csf.25.269

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(27)Cited-By(1)We prepared a cell model of Amoeba proteus by mechanical bursting to study the interaction between actin filaments (AFs) and plasma membrane (PM). The cell model prepared in the absence of Ca2 + showed remarkable contraction upon addition of ATP. When the model was prepared in the presence of Ca2 +, the cytoplasmic granules formed an aggregate in the central region, having moved away from PM. Although this model showed contraction upon addition of ATP in the presence of Ca2+, less contraction was noted. Staining with rhodamine-phalloidin revealed association of AFs with PM in the former model, and a lesser amount of association in the latter model. The interaction between AFs and PM was also studied using the isolated PM. AFs were associated with PM isolated in the absence of Ca2+ , but were not when Ca2+ was present. These results suggest that the interaction between AFs and PM is regulated by Ca2+.

【 授权许可】

Unknown