Cell Structure and Function | |
Insights into the Molecular Mechanism of Nuclear Trafficking Using Nuclear Transport Factor 2 (NTF2) | |
Murray Stewart1  | |
[1] Medical Research Council Laboratory of Molecular Biology | |
关键词: nuclear trafficking; nucleoporins; structure; nuclear pores; | |
DOI : 10.1247/csf.25.217 | |
学科分类:分子生物学,细胞生物学和基因 | |
来源: Japan Society for Cell Biology | |
【 摘 要 】
References(71)Cited-By(14)Nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP. The simplicity and specialization of this system, combined with the availability of crystal structures of NTF2, RanGDP and their complex, has facilitated the investigation of the molecular mechanism of its trafficking. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins. Mutants engineered on the basis of structural information together with determination of binding constants have been used to dissect the roles of these interactions in transport. Thus, NTF2 binds to RanGDP sufficiently strongly for the complex to remain intact during transport through NPCs, but the interaction between NTF2 and FxFG nucleoporins is much more transient, which would enable NTF2 to move through the NPC by hopping from one repeat to another. An analogous nucleoporin hopping mechanism may also be used by carrier molecules of the importin-β family to move through NPCs.
【 授权许可】
Unknown
【 预 览 】
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RO201912080704884ZK.pdf | 1554KB | download |