Induction of Apoptosis by Coprinus disseminatus Mycelial Culture Broth Extract in Human Cervical Carcinoma Cells

Cell Structure and Function

Takao Shinozawa²  Tetsuo Toyomastu¹  Bingye Han² 

[1] The Mushroom Research Institute of Japan, Kiryu, Gunma 376-0051, Japan;Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan

关键词: apoptosis;    Coprinus disseminatus;    mycelial culture broth;    caspase-3;   

DOI  :  10.1247/csf.24.209

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(21)Cited-By(4)Extract of Coprinus disseminatus (pers. Fr.) (C. disseminatus) culture broth (EDCB) inhibits proliferation and induces apoptosis in the human cervical carcinoma cells at 5 μg/ml. To determine whether the cell death induced by the EDCB recruits caspases or not, one of the exclusive pathways in cell death, we examined caspase-3 activity in this cell death process. The activity of caspase-3 was remarkably increased when the cell was treated with EDCB, and this activity was nullified by Z-VAD-FMK, a well known caspase-3 inhibitor. From then results, we would expect the EDCB to contain substances with the ability to induce apoptosis in the human cervical carcinoma cells. The extent of the EDCB induced apoptosis is cell line-dependent.

【 授权许可】

Unknown