Establishment and Characterization of Tracheal Epithelial Cell Lines, TM01 and TM02-3, from Transgenic Mice Bearing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene

Cell Structure and Function

Kotaro Ishibashi²  Fumio Numata⁴  Norifumi Sugiyama⁵  Shinetsu Ono⁴  Yoshiaki Tabuchi³  Masuo Obinata¹  Mitsuru Furusawa⁵  Tadashi Horiuchi⁵  Yoko Uchida⁵ 

[1] Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University;Experimental Technology Research Center, Daiichi Pharmaceutical Co., Ltd.;Basic Technology Research Laboratory, New Product Research Laboratories III, Daiichi Pharmaceutical Co., Ltd.;Basic Technology Research Laboratory, New Product Research Laboratories IV, Daiichi Pharmaceutical Co., Ltd.;Basic Technology Research Laboratory, New Product Research Laboratories, Daiichi Pharmaceutical Co., Ltd.

关键词: tracheal epithelial cell line;    transgenic mouse;    temperature-sensitive growth;    high molecular weight glycoconjugates;    intracellular adhesion molecular-1;   

DOI  :  10.1247/csf.23.119

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(37)Cited-By(8)Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact with neighboring cells, and retained the characteristics of epithelial cells with microvilli on the surface. These cells grew at a permissive temperature (33°C), but did not at a nonpermissive temperature (39°C), indicating that TM01 and TM02-3 cells undergo temperature-sensitive growth. Large T-antigen was expressed only in the nuclei at 33°C. Sepharose CL-4B column chromatography using a 14C-glucosamine hydrochloride, indicating that both cells produced high molecular weight glycoconjugates, and suggesting that these cells may originate from mucus-producing cells. TM01 cells expressed intercellular adhesion molecular-1 (ICAM-1) in both unstimulated and stimulated (1, 000 U/ml tumor necrosis factor-a and 500 U/ml interferon-γ) conditions, whereas TM02-3 cells expressed ICAM-1 only under stimulated conditions. We conclude that these cell lines may serve as a useful model to study the tracheal cell functions under defined in vitro conditions.

【 授权许可】

Unknown