Nuclear Translocation of the Catalytic Component of DNA-dependent Protein Kinase upon Growth Stimulation in Normal Human T Lymphocytes

Cell Structure and Function

Fumiaki Watanabe²  Hirobumi Teraoka²  Kinji Tsukada²  Masayuki Nagasawa⁴  Akira Suwa³  Kohtaro Yamamoto¹ 

[1] Department of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University;Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University;Division of Rheumatic Diseases, Tokyo Metropolitan Ohtsuka Hospital;Department of Pediatrics, School of Medicine, Tokyo Medical and Dental University

关键词: DNA-dependent protein kinase;    Ku protein;    cell cycle;    T lymphocytes;    nuclear localization;   

DOI  :  10.1247/csf.22.585

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(38)Cited-By(12)DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human peripheral blood mononuclear cells, DNA-PK activity and the protein level of DNA-PKcs were very low in the nuclear extracts, but they were high in the whole cell extracts. Depending upon proliferation of the T lymphocytes, DNA-PK activity and the protein level of DNA-PKcs in the nuclear extracts greatly increased. Immunocytochemical analysis suggested translocation of DNA-PKcs from the cytoplasm to the nucleus upon growth stimulation in the T lymphocytes.

【 授权许可】

Unknown