A Novel Truncated Variant Form of Ebk/MDKl Receptor Tyrosine Kinase Is Expressed in Embryonic Mouse Brain

Cell Structure and Function

Takashi Muramatsu¹  Amjad H. Talukder¹  Norio Kaneda¹ 

[1] Department of Biochemistry, Nagoya University School of Medicine

关键词: Eph/Eck family;    PCR Cloning;    Receptor Tyrosine Kinase;    Splice Variant;    Truncated Form;   

DOI  :  10.1247/csf.22.477

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(36)Cited-By(1)We have isolated cDNA clones from a mouse embryonic head cDNA library that encode one member of the Eph/Eck family of receptor tyrosine kinases (RTKs), Ebk/MDKl. Among the 10 clones, two showed full-length type comprising extracellular, transmembrane and intracellular kinase domains. Two of them were modified just after the transmembrane domain and stop codon appeared before completing the kinase domain. This truncated form also had a deletion of five amino acids at the extracellular domain, indicating that it is a novel variant of Ebk/MDKl. RNase protection assay showed that this truncated deleted type, named Ebk-tdl, is present in the head of embryos, although the amount is less compared to that of the full-length type having a deletion of four amino acids. Considering the source and expression of Ebk/MDKl mRNAs, they may have an important role, accompanied with a possible regulatory role of the truncated variant, during neural development and/or in embryogenesis.

【 授权许可】

Unknown