Characterization of The Carrot β-Tubulin Gene Coding a Divergent Isotype, β-2

Cell Structure and Function

Kazuhiko Sonehara¹  Tomoaki Kataoka¹  Shioko Kuramori¹  Maki Tatsuta¹  Keiko Naito¹  Hiromi Ohkawa¹  Mikado Minamizono¹  Shoji Okamura¹ 

[1] Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

关键词: β-tubulin isotypes;    carrot;    colchicine;    hydrophobicity profile;    gene expression;   

DOI  :  10.1247/csf.22.291

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(32)Cited-By(1)Four different β-tubulin clones were isolated from carrot genomic and cDNA libraries. Their nucleotide sequences were determined1 and their predicted amino acids were compared with each other. The predicted amino acid composition of the C-terminal region of three of them (β-1, 3, 4) resembled one another, but that of one isotype (β-2) was divergent. The β-2 tubulin included two hydroxyl amino acids, serine and threonine, and consisted of a lower number of negatively charged amino acids than the others in the C-terminal region. The predicted hydrophobicity profile of the β-2 tubulin around the residue 200 is less hydrophobic than β-1, but it is still more hydrophobic than those of animal and fungal β-tubulins. The β-2 gene was transcribed in cultured cells and flowers, while the β-1 gene was ubiquitously transcribed in cultured cells, roots, shoots and flowers. When the predicted amino acids of plant tubulin were compared with those of other organisms, substitutions from non-polar amino acids to those with hydroxyl group were conspicuous in the region corresponding to the third exon in the plant genes.

【 授权许可】

Unknown