Molecular Characterization of Extrachromosomal Circular DNAs from Differentiating Embryonic Stem Cells

Cell Structure and Function

Yoichiro Iwakura¹  Hideo Yamagishi²  Hiroshi Arakawa²  Takeyuki Shimizu² 

[1] Institute of Medical Science, University of Tokyo;Department of Biophysics, Graduate School of Science, Kyoto University

关键词: E14 ES cell;    differentiation;    circular DNA;    cloning;    breakpoint;    short inverted repeat;   

DOI  :  10.1247/csf.21.451

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(39)Cited-By(1)Embryonic E14 stem cells were differentiated to parietal yolksac-like flat cells in vitro in the absence of added feeders and LIF (Leukemia Inhibitory Factor). We cloned circular DNAs from the differentiating E14 cells. Out of 9 DNA inserts with the unique sequence, one clone showed a chromosomal rearrangement which could have occurred between a pair of short inverted repeats. Recombination mechanism is discussed in view of two other circularization events of the flanking sequences between short inverted repeats shown in differentiated P19 embryonal carcinoma cells.

【 授权许可】

Unknown