Morphological Patterns and DNA Polymerase Regulation in Apoptotic HL60 Cells

Cell Structure and Function

Elisabetta Falcieri²  Angela di Baldassarre²  Amelia Cataldi²  lole Robuffo¹  Sebastiano Miscia² 

[1] Istituto di Citomorfologia Normale e Patologica;Istituto di Morfologia Umana Normale, Università di Chieti

关键词: apoptosis;    ultrastructure;    nick translation;    DNA electrophoresis;    DNA polymerases;   

DOI  :  10.1247/csf.21.213

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(39)Cited-By(6)An ultrastructural and functional study was performed during methotrexate (MTX) induced apoptosis on HL-60 leukemia cells. The fine preservation of plasma membrane architecture and organellar components was present untill latest apoptotic stages, despite strong nuclear changes. This observation suggests the presence of a residual cell metabolic activity, which is here investigated. DNA agarose gel electrophoresis demonstrated its fragmentation, which was also confirmed in situ by nick translation confocal analysis. Nuclear purification was subsequently performed to investigate DNA polymerase activities. DNA polymerase α activity appeared strongly reduced from the early phases of methotrexate treatment, while the rate of DNA polymerase β synthesis was found to increase progressively along with the time of drug treatment. Low levels of DNA polymerase γ activity were revealed both in control and in treated cells, suggesting the irrelevant involvement of this enzyme in the DNA metabolism of this model. DNA polymerase β appears thus to be the enzyme prominently correlated to cell attempts to repair the methotrexate-induced DNA damage.

【 授权许可】

Unknown