Effects of Sulphydryl Reagents on Receptor-Mediated Hormonal Responses at the Cellular Level: Insulin-mimicking Characteristics of Thiol-blocking Compounds in Rat Hepatocyte Primary Cultures

Cell Structure and Function

Boot J. H.¹ 

[1]Department Veterinary Pharmacology, Pharmacy and Toxicology

关键词: SH;    thiol-blocking compounds;    endocrine test-system;    rat hepatocyte primary cultures;    insulin-mimicking;    in vitro toxicology;   

DOI  :  10.1247/csf.21.1

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(42)Cited-By(1)The interference of various SH-blocking chemicals with the insulin-controlled regulation of the hepatic carbohydrate metabolism was studied in rat hepatocyte primary cultures. The organic mercurials PCMB, PCMBS, mersalyl the disulphide agents DTP, CPDS, disulfiram and the SH-alkylating reagent NEM were used as experimental SH-blocking model compounds. All studied compounds, except for NEM, induced an increased glycogen deposition comparable with the physiological insulin-induced glycogen-deposition. PCMBS appeared to be the most effective insulin-mimicking anabolic trigger. The action of the insulin molecule itself was potentiated by PCMBS as well as demonstrated by increased glycogen deposition, induced pyruvate kinase (PK) and decreased phosphoenol-pyrnvate carboxykinase (PEP-CK) activity. However, cell-exposure to insulin and PCMBS in relatively high doses was destructive, as demonstrated by decreased glycogen levels, most probably as a result of insulin-receptor overstimulation and metabolic stress. Thus, SH-blocking compounds are able to trigger insulin-dependent metabolic processes. The relatively non-permeant organic mercurial PCMBS proved to be the most effective insulin-mimicking SH-blocking compound.

【 授权许可】

Unknown