Artificial Cell-spreading of Permeabilized BHK Cells on Fibronectin-coated Substratum

Cell Structure and Function

Michiko Ishikawa-Sakurai¹  Takako Hamano¹  Masao Hayashi¹  Yasunori Miyamoto¹ 

[1] Department of Biology, Ochanomisu University

关键词: cell model;    cell attachment;    cell-spreading;    artificial cell;    fibronectin;    saponin;   

DOI  :  10.1247/csf.20.311

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(26)We developed a cell-spreading model of permeabilized BHK cells to analyze molecular interactions in the cell-spreading machinery. BHK cells in suspension were allowed to attach onto the fibronectincoated cover slips at 37°C for 20 min. The cells were then permeabilized with 50 μg/ml saponin for 10 min at 4°C. Approximately 50 percent of the cell models spread in response to exogenous 1 Mm ATP for 90 min at 37°C. Anti-actin antibody penetrated into almost all the cell models which spread ATP-dependently. These indicate that half number of the cell models permeabilized with saponin is spread by the exogenous ATP and has pores larger than an IgG molecule.

【 授权许可】

Unknown