Quantitative Cryoimmunogold Electron Microscopic Studies on Induction of Serine : pyruvate Aminotransferase in Rat Liver Mitochondria by Administration of Glucagon

Cell Structure and Function

Akitsugu Yamamoto²  Koji Iwata¹  Toshiaki Setoguchi¹  Kyu-Ichiro Okuda¹  Yutaka Tashiro²  Toshiaki Oda³  Syushi Higashi¹  Shingo Satoh¹ 

[1] Department of Surgery I, Miyazaki Medical College;Department of Physiology, Kansai Medical University;Department of Biochemistry, Hamamatsu University School of Medicine

关键词: quantitative immunogold electron microscopy;    cryoultrathin section;    serine : pyruvate aminotransferase;    rat liver;    mitochondria;   

DOI  :  10.1247/csf.20.89

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(26)Induction of mitochondrial serine : pyruvate aminotransferase (SPT) in rat liver by administration of glucagon was studied quantitatively by immunoblot analysis and cryoimmunogold electron microscopy.Immunoblot analysis revealed that two daily injections of glucagon produced marked increase of SPT protein mass to a level as much as 18 times that of the untreated rat. Cryoimmunogold electron microscopic analysis showed that the labeling density of the mitochondria increased in a parallel manner. Thus the induction of SPT analyzed by two methods showed an excellent correlation with a relative correlation coefficient of 0.98, indicatingthat the induction of SPT can be analyzed quantitatively by immunogold electron microscopy on cryoultrathin sections.

【 授权许可】

Unknown