Isolation of Flat Revertants from Human Papillomavirus Type 18 E6E7 Transformed 3Y1 Cells by Transfection with a Rat Embryo Fibroblast cDNA Expression Library

Cell Structure and Function

Kazuyoshi Nakanishi¹  Naoto Hayasaka¹  Hiroshi Nojima³  Hwang Yong-Il¹  Yasunari Takami²  Haruko Ishiwatari¹  Akira Hakura¹  Masuo Yutsudo¹ 

[1] Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University;Department of Biochemistry, Miyazaki Medical College;Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University

关键词: human papillomavirus;    transformation;    cDNA expression library;    revertants;   

DOI  :  10.1247/csf.18.457

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(29)Cited-By(1)A rat embryo fibroblast (REF) cDNA expression library was transfected into 3Y1 cells transformed by human papillomavirus type 18 E6 and E7 genes and 10 flat revertants were isolated. These revertants expressed the same levels of E6 and E7 mRNA as the parent cells, but had greatly reduced ability to form colonies in soft agar. Suppression of transformation was dominant in cell hybrids generated by fusing each revertant with the parental transformed cells. Furthermore, loss of transfected cDNA was observed in re-transformed cell hybrids derived from one flat revertant. Over expression of the CDNA suppresses the colony-forming efficiency of the cells transformed by E6 and E7 genes.

【 授权许可】

Unknown