Cell Structure and Function | |
Isolation of Flat Revertants from Human Papillomavirus Type 18 E6E7 Transformed 3Y1 Cells by Transfection with a Rat Embryo Fibroblast cDNA Expression Library | |
Kazuyoshi Nakanishi1  Naoto Hayasaka1  Hiroshi Nojima3  Hwang Yong-Il1  Yasunari Takami2  Haruko Ishiwatari1  Akira Hakura1  Masuo Yutsudo1  | |
[1] Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University;Department of Biochemistry, Miyazaki Medical College;Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University | |
关键词: human papillomavirus; transformation; cDNA expression library; revertants; | |
DOI : 10.1247/csf.18.457 | |
学科分类:分子生物学,细胞生物学和基因 | |
来源: Japan Society for Cell Biology | |
【 摘 要 】
References(29)Cited-By(1)A rat embryo fibroblast (REF) cDNA expression library was transfected into 3Y1 cells transformed by human papillomavirus type 18 E6 and E7 genes and 10 flat revertants were isolated. These revertants expressed the same levels of E6 and E7 mRNA as the parent cells, but had greatly reduced ability to form colonies in soft agar. Suppression of transformation was dominant in cell hybrids generated by fusing each revertant with the parental transformed cells. Furthermore, loss of transfected cDNA was observed in re-transformed cell hybrids derived from one flat revertant. Over expression of the CDNA suppresses the colony-forming efficiency of the cells transformed by E6 and E7 genes.
【 授权许可】
Unknown
【 预 览 】
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RO201912080704519ZK.pdf | 1616KB | download |