Differentiation and Dedifferentiation of the Human Monocytic Leukemia Cell Line, U937

Cell Structure and Function

Tohru Marunouchi¹  Hiromi Hosoya¹ 

[1]Division of Cell Biology, Institute for Comprehensive Medical Science, Fujita Health University

关键词: differentiation;    dedifferentiation;    leukemia;    macrophage;    U937;   

DOI  :  10.1247/csf.17.263

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(14)Cited-By(10)U937 cells were differentiated into macrophages after being treated with 12-o-tetradecanoylphorbol- 13-acetate (TPA) for the first two days and dedifferentiated with daily medium renewal for 10 days. Cell proliferation slowed down and the number of cells reached the maximum level on day 2. By day 4, all of the cells had spread and attached firmly to the culture dish, and more than 90% of the cells expressed the Fc-receptor and produced superoxide anion. From there on, the number of adherent, living cells decreased gradually to about half the initial count. Most of the cells eliminated from the culture by cell death were in the S phase at the time of TPA treatment. After day 8, the number of cells expressing macrophage-specific phenotypes gradually decreased, cell adhesion was weakened, and at the same time, DNA synthesis was initiated anew. The cells became round and began to proliferate as floating cells on days 9 to 10, and thereafter they became sensitive to the second round of TPA treatment. On the basis of all the results taken together, it is suggested that fully differentiated U937 cells were dedifferentiated after being cultured with frequent medium renewal.

【 授权许可】

Unknown