An Antigenic Determinant on Human Centromere Protein B (CENP-B) Available for Production of Human-Specific Anticentromere Antibodies in Mouse

Cell Structure and Function

Kenji Sugimoto¹  Hideyuki Migita¹  Michio Himeno¹  Hiroaki Yata¹  Yoshimasa Hagishita¹ 

[1] Laboratory of Biochemistry, Department of Agricultural Chemistry, University of Osaka Prefecture

关键词: Centromere protein B (CENP-B);    antigenic determinant;    anticentromere antibodies;    epitope mapping;    mammalian cells;   

DOI  :  10.1247/csf.17.129

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(27)Cited-By(12)Centromere protein B (CENP-B) is one of the centromere DNA binding proteins constituting centromere heterochromatin throughout the cell cycles. Some components of mammalian centromeres including CENP-B are target antigens for autoimmune disease patients, often those with scleroderma. Recent isolations of CENP-B genes from human and mouse suggested that CENP-B was highly conserved among mammals. From the previous analysis of the reactivity of patient anticentromere sera, two autoepitopes have been located on the DNA binding domain at the amino-terminal region. The amino acid sequences for both the epitopes are perfectly conserved in the two species, human and mouse. In this study, to identify a human-specific antigenic determinant, the remaining two epitopes were further located in separate carboxyl-terminal regions of human CENP-B. Although the amino acid sequence of one epitope is identical to that of the corresponding region in mouse CENP-B, the other has a less homologous sequence. To confirm that the latter epitope was available for production of human-specific anticentromere antibodies, mice were immunized with the recombinant human CENP-B product. One serum that exclusively stained human centromere structure, but not that of other mammals, was identified in the immunofluorescence microscopic observation. The epitope analysis showed that the less conserved one was recognized by this serum. These results suggested that the corresponding region defines the antigenic determinants for the species specificity.

【 授权许可】

Unknown