Characterization of Lipoprotein Secreted by Cultured Eel Hepatocytes and its Comparison with Serum Lipoproteins

Cell Structure and Function

Fu-gong Yu¹  Seiichi Ando¹  Seiichi Hayashi¹ 

[1]Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University

关键词: apolipoprotein;    eel;    hepatocytes;    lipoproteins;    VLDL;   

DOI  :  10.1247/csf.16.347

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(35)Cited-By(17)The lipoprotein secreted by cultured eel hepatocytes was fractionated by density gradient ultracentrifugation and compared with eel serum lipoproteins. Eel hepatocytes were cultured for 7 to 10 days as a monolayer in Williams' medium E containing 5 % fetal bovine serum and 0.16 μM insulin on a dish precoated with fibronectin of horse serum. The only lipoprotein secreted by eel hepatocytes was a very-low-density lipoprotein like one which consisted of 69 % triglyceride, 15 % phospholipid, 4 % cholesterol, and 12 % protein. On the other hand, very-low-density lipoprotein and high density lipoprotein were found in eel serum, in which high density lipoprotein was a main lipoprotein. The secreted lipoprotein contained apo B and apo A as the main protein components. Furthermore, the lipoprotein contained proapo A-I in addition to apo A-I, which was proved by comparing the amino acid composition of both proteins. In our discussion, we noted that the lipoprotein secreted by eel hepatocytes was a good material for the study of high-density lipoprotein formation.

【 授权许可】

Unknown