Imaging of Calcium Wave Propagation in Guinea-Pig Ventricular Cell Pairs by Confocal Laser Scanning Microscopy

Cell Structure and Function

Tetsuro Takamatsu¹  Setsuya Fujita¹  Hideyuki Kawachi¹  Tetsuhiro Minamikawa¹ 

[1] Department of Pathology, Kyoto Prefectural University of Medicine

关键词: confocal laser scanning microscope;    fluorescence;    ventricular cell pair;    calcium wave;    fluo-3;   

DOI  :  10.1247/csf.16.341

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(18)Cited-By(13)We describe here the use of a confocal laser scanning microscope for imaging fast dynamic changes of the intracellular calcium ion concentration ([Ca2+]i) in isolated ventricular cell pairs. The scanning apparatus of our system, paired galvanometer mirrors, can perform narrow band scanning of an area of interest at a high temporal resolution of less than 70 msec per image. The actual [Ca2+]i is obtained directly through the fluorescence intensity of injected fluo-3, which responds to changes of [Ca2+]i in optically sectioned unit volumes of the cell. Images of the calcium wave obtained during propagation between paired cells revealed that the wave front is contant in shape and propagates at constant velocity without any delay at the cell-to-cell junction. The confocal laser scanning microscope with depth-discriminating ability is a valuable tool for taking pictures of the sequence of biological events in living cells.

【 授权许可】

Unknown