Quantification of Cell Nuclei Isolated from Hepatocytes by Cell Lysis with Nonionic Detergent in Citric Acid

Cell Structure and Function

Yoshifumi Horiuti¹  Kazuo Yano¹  Yuzo Shibuya¹  Motohiro Ogishima¹ 

[1] Research Laboratories, Toyo Jozo Co., Ltd.

关键词: quantification;    hepatocyte;    cell nuclei;    detergent;    nikkol BO-10TX;   

DOI  :  10.1247/csf.16.203

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(9)Cited-By(27)A method was developed for determining the number of nuclei of hepatocytes cultured on collagen gel using a nonionic detergent, Nikkol BO-10TX. The cells were recovered in a test tube after solubilizing the gel by incubating it with the detergent in 0.1 M citric acid and then centrifuging the mixture. Nuclei were isolated from the cells with the same detergent solution and collected by centrifugation. The numbers of nuclei in cultures, scored with a hemocytometer or an electronic particle counter, were proportional to the lactate dehydrogenase activities of the cells. This method was also applicable for scoring the number of nuclei of hepatocytes cultured on collagen-coated plastic.

【 授权许可】

Unknown